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【目的】本试验以同德老芒麦和DJ-01老芒麦的种子为试验材料,探究2份老芒麦种子萌发期的抗旱性。【方法】试验采用-0.1 MPa、-0.3 MPa、-0.5 MPa、-0.7 MPa、-1.0 MPa的高分子渗透剂聚乙二醇-6000(PEG-6000)溶液模拟干旱胁迫,以蒸馏水为对照组(CK),测定两份老芒麦种子的发芽指标及其抗旱系数。【结果】当胁迫水势低于-0.1 MPa时,老芒麦种子发芽率降至22%以下,发芽势降至10%以下;当胁迫水势低于-1.0 MPa时,老芒麦种子均不萌发;当胁迫水势为-0.1~-0.3 MPa时,利于老芒麦胚根的生长。【结论】干旱胁迫均抑制了两份老芒麦种子的萌发,且随着干旱胁迫强度的增强,其发芽率、发芽势、相对发芽率、相对发芽势等均呈现下降趋势;当胁迫水势较低时,干旱胁迫对老芒麦胚根的生长具有促进作用。
Abstract:【Objective】In this experiment, 'Tongde' Elymus sibiricus and 'DJ-01' Elymus sibiricus were used as test materials to investigate the drought tolerance during the germination period.【Methods】The experiment was conducted to simulate drought stress by using -0.1,-0.3,-0.5,-0.7, and -1.0 MPa of polymer osmotic agent polyethylene glycol-6000(PEG-6000), and distilled water as the control group(CK) to determine the germination indexes of the two Elymus sibiricus germplasm materials and their drought coefficients.【Results】When the stress water potential was lower than -0.1MPa, the germination rate of Elymus sibiricus materials decreased to less than 22%, and the germination potential decreased to less than 10%; when the stress water potential was lower than -1.0MPa, none of the Elymus sibiricus material germinated; when the stress water potential was -0.1 ~-0.3MPa, it was favorable to the germinal root growth of Elymus sibiricus.【Conclusion】Drought stress inhibited the germination of all the Elymus sibiricus materials, and showed a decreasing trend of germination rate, germination potential, relative germination rate, relative germination potential and other indexes of Elymus sibiricus with the increase of the intensity of drought stress; when the stress water potential was lower, the drought stress had a favorable effect on the growth of Elymus sibiricus radicle.
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基本信息:
DOI:10.16863/j.cnki.1003-6377.2023.05.008
中图分类号:S543.9
引用信息:
[1]徐婉宁,孙群策,张树振等.两份老芒麦种质材料萌发期抗旱性评价[J].草食家畜,2023,No.222(05):45-50.DOI:10.16863/j.cnki.1003-6377.2023.05.008.
基金信息:
国家牧草产业技术体系项目(CARS-34); 青藏高原种质资源研究与利用实验室开放课题(2023-SYS-05)