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本试验选用12头24月龄杂交肉牛(利木赞×鲁西黄牛,平均体重为532.6±20.9kg)作为试验牛,研究了不同水平的热处理大豆对肉牛背最长肌、背部脂肪、肝脏和血液中类胰岛素生长因子-Ⅰ和生长激素的影响。试验牛被随机分为三组,每组包括4头动物,试验日粮分别含有3.60%、10.06%和15.72%的粗粉碎热处理大豆,记为处理1、处理2和处理3。试验期为28d,试验结束后采集背最长肌、背部脂肪、肝脏和血液样品,测定类胰岛素生长因子-Ⅰ、瘦素、生长激素和胰岛素水平。试验结果表明,三个处理组肝脏和背部脂肪中的类胰岛素生长因子-Ⅰ水平之间没有显著差异(P>0.05);添加10.06%大豆组的背最长肌的胰岛素样生长因子-Ⅰ水平显著高于其他两个处理组(P<0.05);三个处理组的肝脏和背部脂肪中的瘦素含量没有显著差异(P>0.05)。处理1和处理2背最长肌中的瘦素水平显著高于处理3(P<0.05)。粗脂肪的采食量与肝脏中IGF-I的水平之间存在显著的二次曲线关系。试验结果说明,如果热处理大豆的添加量恰当(在本试验中为10.06%),可以提高背最长肌中的IGF-Ⅰ水平,进而提高肉牛生产性能。试验还发现,粗脂肪采食量是影响肉牛肝脏中IGF-Ⅰ平的最主要因素之一,二者存在显著二次相关关系(R2=0.775)。
Abstract：Twelve 24-month-old castrated crossbred bulls(Luxi×Limousin) weighing 532.6±20.9 kg were used to study the effect of feeding different levels of soybeans on different hormones in longissimus dorsi muscle,back fat,liver and serum of beef cattle.The cattle were divided into 3 groups with 4 cattle in each group and were fed diets containing 3.60%,10.06% and 15.72% of coarsely ground roasted soybeans as Treatments 1,2 and 3,respectively.The cattle were slaughtered at the end of the 28-day feeding experiment.Tissue and serum samples were obtained for analysis of IGF-I,leptin,growth hormone and insulin.The results showed that no difference was found between the levels of IGF-I in liver and back fat of all the treatments(P>0.05).The level of IGF-I in longissimus dorsi muscle of Treatment 2 was significantly higher than that of Treatment 1 and Treatment 3(P<0.05) while no difference was found in the levels of leptin in liver and back fat of all the treatments(P>0.05).The level of leptin in longissimus dorsi muscle of Treatment 1 and Treatment 2 was significantly higher than that of Treatment 3(P<0.05).A significant quadratic relationship between the intake of ether extract and the level of IGF-I in liver was found.The results indicated that the level of IGF-I in longissimus dorsi muscle can be enhanced if the supplementation level of roasted coarsely-ground soybean is proper(namely,10.06% in this article).Thus the production performance of beef cattle can be improved.In addition,the experiment indicated that the intake of ether extract was one of the most important factors affecting the level of IGF-I in the liver of beef cattle.
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基本信息:
DOI：10.16863/j.cnki.1003-6377.2011.02.013
中图分类号:S823.5
引用信息:
[1]孙伟,马文健,宋恩亮等.饲喂不同水平热处理大豆对肉牛体组织和血清中类胰岛素生长因子-Ⅰ、生长激素、胰岛素和瘦素水平的影响[J].草食家畜,2011,No.151(02):35-39.DOI:10.16863/j.cnki.1003-6377.2011.02.013.
基金信息:
“十一五”科技支撑计划项目(编号:2006BAD14B07-5-3); 西部优质肉牛最优化饲料饲技术体系的建立和验证(编号:NYHYZX-07-035-05)