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为优化牛体外培养系统,本研究利用微滴培养法,探讨了卵巢状态所获卵母细胞、种公牛个体、受精时间、季节及共培养对体外培养结果的影响。结果表明:(1)不同卵巢状态所获卵母细胞的分裂率、囊胚率差异均不显著(P>0.05);(2)不同种公牛精液进行体外受精,结果显示分裂率和囊胚率差异显著(P<0.05);(3)6h、8h、12h和16h受精时间比较,结果以12h效果最佳,分裂率、囊胚率与其它各组均差异显著(P<0.05)。(4)春、秋季体外培养的效果显著优于夏、冬季。(5)使用颗粒单层可显著提高囊胚率(P<0.05)。
Abstract：The paper discussed the effects of oocytes derived from slaughterhouse different states of ovaries,different semen,fertilization time,season and co-culture for in vitro culture of bovine with droplet method.(1) After culture the oocytes derived from slaughterhouse different states of ovaries,there were no significant difference in cleavage rate and blastocyte rate.(2) There were significant difference in cleavage rate and blastocyte rate in different semen.(3) The effect on fertilization time of 12h is better.It had significant difference compare to 6h、8h and 16h.(4) Spring and autumn had better effect than summer and winter on in vitro fertilization.(5) Granular cell layer can improve the blastocyte rate obviously,but can not improve the cleavage rate.
[1]Lu K H,Gordon I,Chen H B,et al.Birth of twins after transfer of cattle embryos produced by in vitro techniques[J].Vet Rec,1988,122:539～540.
[2]Hazeleger N L,Hill DJ,StubbingR B,et al.Relat-ionship of morphology and follicular fluid environm-ent of bovine oocyte to their developmental potential in vitro[J].Theriogenology,1995,(43):509～522.
[3]Behboodi E,Anderson GB,Bondurant RH.Develo-pment of in vitro fertilization oocytes from pregnant and nonpregnant cows in oviductal epithelial and cumulus cell co～culture systems[J].Theriogenology,1992,(38):1077～1084.
[4]陈静波.牛妊娠期黄体形态结构及卵母细胞体外胚胎生产的研究[D],西北农林科技大学博士论文,2003.
[5]王锋,牛羊繁殖学[M].北京:中国农业出版社.
[6]Rehman N,Collins A R,Suh T K,et al.Effect of sperm exposure time on in vitro fertilization and embryo development of bovine oocytes matured invitro[J].Theriogenology,1994,(41):1447～21452.
[7]孙青原,秦鹏春,刘国艺,等.牛体外受精的程序及超微结构研究[J].动物学报,1996,42(3).
[8]文国艺,卢克焕.受精液中肝素和咖啡因的组合对牛卵母细胞体外受精的影响[J].广西农业大学学报,1994,13(1):27231.
[9]乐杰.妇产科学第5版[M].北京:人民卫生出版社,2002,34～351.
[10]罗光彬等,体外受精温度、时间及卵子质量对延边黄牛受精卵体外发育的影响[J].中国畜牧兽医,2004,(1).
[11]Dirnfeld M,Bider D,Koifman M,et al.Shortened exposure of oocytes to spermatozoa improves in vitro fertilization outcome:a prospective randomized controlled study[J]Hum Re prod,1999,(14):2562～2564.
[12]Smith SD,Mikkelsen A Lindenberg S.Development of human oocytes matured in vitro for28or36hou-rs[J].Fertil Steril,2000,73(3):541～544.
[13]SirardM,Parrish J,Ware C,et al.The culture of bovine oocytes to obtain developmentally competent embryos[J].Bio Rep rod,1989,(40):720～728.
[14]Host E,Mikkelsen AL,Lindenberg S,et al.Apopt-osis in human cumulus cells in relation to matur-ation stage and cleavage of the corresponding oocyte[J].Acta Obstet Gynecol Scand,2000,79(11):936～940.
[15]Eppig JJ.Growth and development of the mammalian oocytes granulosa cell complex in culture.In:Trounson Gosden RG(eds).Biology and Pathology of Oocyte.Cambridge:Cambridge University Press,2003,p.273～282.
基本信息:
DOI：10.16863/j.cnki.1003-6377.2007.02.010
中图分类号:S823
引用信息:
[1]张金花,海里且木,陈静波.优化牛体外生产胚胎条件的研究[J].草食家畜,2007,No.135(02):27-30.DOI:10.16863/j.cnki.1003-6377.2007.02.010.
基金信息:
新疆科技攻关项目《肉奶牛胚胎产业化及高效繁育体系的建立》,项目编号:200131102