采用假阴道法采集成年种公驴精液,经品质评定后,用5种不同冷冻保存液稀释、平衡,冷冻保存制作细管冷冻精液,以筛选适宜的驴精液冷冻保存液和冷冻保护剂。结果表明:乳糖-葡糖-甘油-卵黄液为最佳种冷冻保存液(P<0.05);甘油为最佳冷冻保护剂,其适宜浓度为5%,精子冻后活率平均为0.36,畸形率≤27.4%,顶体完整率82.2%,解冻后常温下精子存活时间为8.2h。

The donkey semen was collected by artificial vagina.The extenders of frozen semen,cryoprotectant and the glycerol concentration were investigale in present study.The results shows that the lactose-glucose-glycerol-yolk extender was the best one in 5 groups(P<0.05).The Glycerol was the better cryoprotective preservative compared with Glycol and DMSO.The optimum Glycerol concentration is 5%(P<0.05).The sperm motility after-thawing was the highest in 5 treatment groups.The semen motility of frozen thawing was up to 0.36,Abnormal sperm after-thawing was 27.4%,acrosome integrity after-thawing was 82.2%,and sperm survival time(25℃) was8.2 h,respectively.