【目的】由产气荚膜梭菌引起的家畜疾病给绵羊养殖业的健康发展带来了巨大损害，为了解哈密市绵羊产气荚膜梭菌的流行情况，以便有针对性地对绵羊产气荚膜梭菌病开展防控。【方法】采集哈密市两县一区重点监测网点和规模养殖场的新鲜绵羊粪便，通过聚合酶链式反应（PCR）方法对绵羊中产气荚膜梭菌的感染情况开展调查，然后运用显微镜镜检“、牛乳发酵”试验和DNA琼脂糖凝胶电泳等技术对检测阳性菌进行形态学与分子生物学鉴定。【结果】研究显示，绵羊粪样中产气荚膜梭菌的总检出率为11.34%；不同地区的阳性感染率不同，阳性率最高为19.04%；在33份阳性样品中，72.73%为A型株，27.27%为D型株，未见B、C、E型。【结论】哈密市的绵羊普遍存在产气荚膜梭菌感染，且A型、D型为该地羊源产气荚膜梭菌的流行菌株，本研究为哈密市产气荚膜梭菌的诊断和防控提供了一定的数据支撑。

【Objective】The disease caused by Clostridium perfringens has brought great losses to the healthy development of sheep breeding industry. In order to investigate the prevalence of Clostridium perfringens in sheep in Hami City, so as to carry out targeted prevention and control of the disease.【Methods】Fresh sheep feces were collected from key monitoring sites and largescale sheep farms in two counties and one district of Hami City, and the infection status of Clostridium perfringens in sheep in Hami was investigated by PCR method. Then morphological and molecular identification of the positive bacteria strains were performed by microscopy, "milk fermentation" test and DNA agarose gel electrophoresis.【Results】The results showed that the total detection rate of Clostridium perfringens in sheep feces was 11.34%. The positive infection rate was different in different regions, and the highest positive rate was 19.04%. Among the 33 positive samples, 72.73% were type A strains, 27.27% were type D strains, and no type B, C and E strains were found. 【Conclusion】 In conclusion, Clostridium perfringens infection was widespread in sheep in Hami City, and type A and D strains were the prevalent ones in this area. Certain data support were provided for the diagnosis and control of Clostridium perfringens in Hami City.