| 142 | 4 | 4 |
| 下载次数 | 被引频次 | 阅读次数 |
利用微卫星标记技术,分析了山西黎城大青羊、吕梁黑山羊、波尔山羊、南江黄羊(黑)、南江黄羊(黄)五个山羊群体的分子水平上的遗传多样性,结果表明:7个微卫星位点均为高度多态位点,可以用于山羊群体遗传多样性的评估;平均多态信息含量(PIC)达0.83410.9026;5个山羊群体平均观察杂合度(0.62580.8629)均低于平均期望杂合度(0.85940.9115),证明5个山羊种群具有丰富的遗传多样性和广泛的遗传基础,但品种内存在着一定程度的近交,尤其波尔山羊近交较严重。以Nei氏标准遗传距离为基础的UPGMA和N-J聚类结果表明:吕梁黑山羊与南江黄羊(黑)首先聚为一类,而没和黎城大青羊先聚;国内四个山羊群体与国外品种波尔山羊亲缘关系较远。
Abstract：The genetic diversity of five goat populations was surveyed using 7 microsatellite loci. The five goat populations included Li -cheng blue goat,lvliang black goat,Boer goat, Nanjiang huang goat (blank), Nanjiang huang goat (yellow). The 7 microsatellite loci showed high polymorphism information content value. They can be used for the genetic diversity evaluation in goat breeds.The mean polymorphism information content value was between 0.8341 0.9026,which indicated that the 5 populations had abundant genetic diversity.The mean observed heterozygosit-ies (0.62580.8629) were lower than their mean expected values (0.85940.9115).It was suggested that the five goat populations were highly genetic polymorphic but somewhat inbred.Boer goat is more serious than them. The UPGMA dendrogram and N-J based on the Nei,s genetic distance indicated that lvliang blank goat and Nanjiang huang goat (blank) were clustered first,but Lvliang blank goat were not clustered with Licheng blue goat first. The four goat populations had the distant relationship with Boer goat.
[1]马月辉,徐桂芳,王端云等.中国畜禽遗传资源信息动态研究[J].中国农业科学,2002,35(5):89-92.
[2]李宁.动物遗传学[M].北京:中国农业出版社,2003.
[3]Takezaki N.Nei M.Genetic distance and reconst-ruction of phylogenetic trees from microsatellite DNA[J].Genetics,1996,144:389-399.
[4]张爱玲,马月辉,李宏滨等.利用微卫星标记分析6个山羊品种遗传多样性[J].农业生物技术学报,2006,14(1):38-44.
[5]金冬雁,黎孟枫译.分子克隆实验指南(第2版).北京:科学出版社。1999.30-32.
[6]Nyamsamba D,Nozawa K N.Genetic relationship among Mongolian native goat populations estimated by blood protein polymorphism[J]small Ruminant Res-earch,2003,47:171-181.
[7]Nei M.Estimation of average heterozygosity and gene-tic distance for a small number of in dividual[J].Gene-tics,1978,89:583-590.
[8]卢纹岱.SPSS for Windows统计分析[M].(第2版).北京:电子工业出版社,2002,9.
[9]Bolstein D,White R L.Sko1ick M,et a1.Construction of a genetic linkage map in man using restruction ragment leugth polymorphisms[J].Animal Journal,l980,32:314-331.
[10]王东劲,侯冠或,王文强,等.雷州山羊和隆林山羊遗传多样性的微卫星分析[J].中国农学通报,2007,23(12):37-41.
[11]苑存忠,王建民,马月辉等.山东省地方绵羊品种微卫星遗传多态性[J].应用生态学报,2006,17(8):1459-1464.
[12]孙允东,马月辉,王建民,等.山东省地方山羊品种群微卫星基因座的遗传多样性[J].家畜生态学报,2006,27(2):19-26.
[13]Hines H.C.,Zikakis J.P.,Haenlein G.F.et a1.Linkage relationships among Loci of Polymorphism in Blood and Milk of Cattle[J].Dairy Sci.,1981,64(1):71—76.
[14]李俊雅.模拟群体中基因座位数对NEI氏遗传距离的影响[A].中国动物遗传育种研究[C].北京:中国农业科技出版社,1997,115-118.
[15]Nei M,Tajima F,Tateno Y.Accuracy of estimated phylogenetic trees from molecular data.[J]Molecular Evolution,1983,19:153.
[16]李步高,杜美红,郭晓红等.Boer山羊与山西地方山羊品种聚类分析[J].山西农业大学学报,2005,25(3):243-246.
[17]毛凤显,皇甫江云,赵有璋等.贵州地方山羊品种遗传背景的微卫星分析[J].畜牧与兽医,2006,38(2):13-15.
基本信息:
DOI：10.16863/j.cnki.1003-6377.2009.01.008
中图分类号:S827
引用信息:
[1]常新建,任有蛇,岳文斌等.五个山羊群体微卫星标记的遗传多样性分析[J].草食家畜,2009,No.142(01):22-27.DOI:10.16863/j.cnki.1003-6377.2009.01.008.
基金信息:
国家“十一五”科技支撑计划项目(2007BAD56B03); 山西省重大科技攻关发展计划招标项目(031041); 山西省科技攻关计划项目(031044)资助。