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试验旨在探究高繁殖力候选基因在和田羊群体的遗传特征,为和田羊多羔类群的选育奠定基础。以不同产羔数的和田羊为研究对象,借助特异性内切酶,对TGFβ/BMP信号通路中GDF9(G1位点)、BMPR1B(FecB位点)与BMP15基因(FecXG位点)进行酶切位点分析。经检测,在和田羊中首次发现GDF9、BMPR1B基因具有多态性,GDF9存在2种基因型(GG、GA),BMPR1B存在3种基因型(++、+B、BB),而BMP15基因无多态性;GDF9、BMPR1B基因的PIC分别为低度多态、中度多态,均处于Hardy-Weinberg平衡状态(P>0.05),可世代稳定遗传;GDF9、BMPR1B基因在和田羊母羊群体中存在4种组合基因型,在公羊群体中存在2种组合基因型,公羊、母羊群体的遗传结构特征可能存在差异。以上结果揭示,BMP15基因不是影响和田羊繁殖性状的候选基因;GDF9、BMPR1B基因有待与和田羊繁殖性状进行关联分析以进一步确认。
Abstract：The experiment aimed to explore the genetic characteristics of candidate genes with high fertility in Hotan sheep population, lay the foundation for the breeding of Hotan Sheep with multiple lambs. In this paper, the RFLP analysis of GDF9 gene(G1 site), BMPR1 B gene(FecB site) and BMP15 gene(FecXGsite) of TGFβ/BMP signaling pathway was carried out by using specific endonuclease. It was first found that GDF9 gene had two genotypes(GG, GA), BMPR1 B gene had three genotypes(++, +B and BB), and there was no polymorphism in BMP15 gene. The PIC of GDF9 and BMPR1 B gene were in low and medium degree polymorphism, respectively, and both of them were in Hardy-Weinberg equilibrium(P>0.05), which meant the two genes could be inherited stably for generations. There were 4 combined genotypes of GDF9 and BMPR1 B gene in ewe group of Hotan sheep and 2 combined genotypes in ram group, indicating that the genetic structure characteristics of ram and ewe group may be different. As a conclusion, BMP15 gene was not the candidate gene affecting the reproductive traits of Hotan sheep, while GDF9 and BMPR1 B gene need to be further confirmed by correlation analysis with reproductive traits.
[1] Liu Q Y, Pan Z Y, Wang X Y, et al. Progress on major genes for high fecundity in ewes[J]. Frontiers of Agricultural Science and Engineering, 2015, 1(4):282.
[2]国家畜禽遗传资源委员会.中国畜禽遗传资源志·羊志[M].北京:中国农业出版社，2011.
[3]吴庭辉，李涛，李树伟.平原型和田羊毛囊KAP4.2基因的克隆与原核表达[J].黑龙江畜牧兽医，2019,(13):11-15+20.
[4] Gorlov I F, Kolosov Y A, Shirokova N V, et al. GDF9 gene polymorphism and its association with litter size in two Russian sheep breeds[J]. Rendiconti Lincei. Scienze Fisiche e Naturali, 2017, 29(1):61-66.
[5] Wang W M, Liu S J, Li F D, et al. Polymorphisms of the ovine BMPR-IB, BMP-15 and FSHR and their associations with litter size in two Chinese indigenous sheep breeds[J]. International Journal of Molecular Sciences, 2015, 16(5):11385-11397.
[6] Liu M N, Zhang K, Xu T M. The role of BMP15 and GDF9 in the pathogenesis of primary ovarian insufficiency[J]. Human fertility(Cambridge, England), 2019,(14):1-8.
[7]王嘉鹏.苏尼特羊GDF9基因关联性分析及其突变对启动子活性的影响[D].呼和浩特：内蒙古大学，2019.
[8]汤继顺.利用转录组测序和蛋白质组学分析筛选绵羊多羔候选基因的研究[D].北京：中国农业科学院，2019.
[9] Barzegari A, Atashpaz S, Ghabili K, et al. Polymorphisms in GDF9 and BMP15 associated with fertility and ovulation rate in Moghani and Ghezel sheep in Iran[J]. Reproduction in Domestic Animals, 2010, 45(4):666-669.
[10] Roy J, Polley S, De S, et al. Polymorphism of fecundity genes(FecB, FecX, and FecG)in the Indian Bonpala Sheep[J]. Animal Biotechnology, 2011, 22(3):151-162.
[11] Liandris E, Kominakis A, Andreadou M, et al. Associations between single nucleotide polymorphisms of GDF9 and BMP15 genes and litter size in two dairy sheep breeds of Greece[J]. Small Ruminant Research, 2012, 107(1):16-21.
[12] Abdoli R, Zamani P, Deljou A, et al. Association of BMPR-1B and GDF9 genes polymorphisms and secondary protein structure changes with reproduction traits in Mehraban ewes[J]. Gene, 2013, 524(2):296-303.
[13]古丽格娜，艾买提·买买提，於建国，等.7种绵羊和4种山羊GDF9基因G1突变检测[J].中国草食动物科学，2015,35(4):1-4.
[14]潘章源，贺小云，刘秋月，等.绵羊GDF9基因m RNA、DNA和调控区序列克隆及其在11个品种中遗传多态性检测[J].畜牧兽医学报, 2016, 47(8):1555-1564.
[15]孙庆，田志龙，刘秋月，等.鲁中肉羊BMPR1B、BMP15和GDF9基因多态性与产羔数关联分析[J].中国草食动物科学，2019,39(4):1-5+10.
[16] Mulsant P, Lecerf F, Fabre S, et al. Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes[J]. Proceedings of the National Academy of Sciences, 2001, 98(9):5104-5109.
[17] Kumar S, Mishra A K, Kolte A P, et al. Screening for Booroola(FecB)and Galway(FecXG)mutations in Indian sheep[J]. Small Ruminant Research, 2008, 80(1-3):57-61.
[18] Guan F, Liu S R, Shi G Q, et al. Polymorphism of FecB gene in nine sheep breeds or strains and its effects on litter size, lamb growth and development[J]. Acta Genetica Sinica, 2006, 33(2):117-124.
[19] Yue Y J, Yang B H, Liang X, et al. Simultaneous identification of FecB and FecXG mutations in Chinese sheep using high resolution melting analysis[J]. Journal of Applied Animal Research, 2011, 39(2):164-168.
[20] Mahdavi M, Nanekarani S, Hosseini S D. Mutation in BMPR-IB gene is associated with litter size in Iranian Kalehkoohi sheep[J]. Animal Reproduction Science, 2014, 147(3-4):93-98.
[21]王旭,巴拉提·买买提伊明. BMPR-IB基因作为多浪羊高繁殖力候选基因的研究[J].新疆农业科学,2010, 47(9):1813-1818.
[22]马海玉,王琼,于茜,等. 3个新疆绵羊品种中BMPR-IB基因的RFLP分析及比较[J].西北农业学报,2015, 24(4):19-24.
[23]李达,孙伟,倪荣,等.绵羊FecB基因遗传多样性及其产羔数的关联分析[J].畜牧兽医杂志, 2012, 31(2):1-5.
[24]代金平,刘武军,米日尼萨汗·库尔班,等.策勒黑羊BMPR-IB基因多态性研究[J].中国畜牧兽医,2012, 39(5):145-147.
[25]乔海生,杨博辉,岳耀敬,等.高原型藏羊种公羊群体BMPR-IB基因多态性检测分析[J].黑龙江畜牧兽医, 2018,(16):56-58.
[26] Wang W M, La Y F, Zhou X, et al. The genetic polymorphisms of TGF beta superfamily genes are associated with litter size in a Chinese indigenous sheep breed(Hu sheep)[J]. Animal Reproduction Science, 2018, 189(15):19-29.
[27]张军霞.绵羊繁殖相关候选基因的表达和SNP扫描及其与产羔数关联性分析[D].兰州：甘肃农业大学，2017.
基本信息:
DOI：10.16863/j.cnki.1003-6377.2020.04.001
中图分类号:S826.2
引用信息:
[1]王琼,马海玉,刘玲玲等.GDF9、BMPR1B与BMP15基因在和田羊群体的遗传特征分析[J].草食家畜,2020,No.203(04):1-8.DOI:10.16863/j.cnki.1003-6377.2020.04.001.
基金信息:
新疆维吾尔自治区重点研发计划子课题(2017B01005-2-1); 国家自然科学基金重点项目(U1603232); 国家重点研发计划课题(2018YFD0502101); 新疆维吾尔自治区研究生科研创新项目(XJ2019G133)