本文对弓形虫病的实验室诊断方法,包括病原学(涂片染色法、动物接种法、卵囊分离法和包囊分离法)、免疫学(直接凝集试验(Direct agglutination test,DAT)、间接血凝试验(Indirecthemagglutination test,IHA)、改良凝集试验((Modified agglutination test,MAT)、酶联免疫吸附试验(Enzyme linked immunosorbent assay,ELISA)、补体结合试验(Complement fixation test,CT)、染色试验(Dye test,DT)、间接荧光免疫试验(Indirect fluorescence antibody test,IFAT))和分子生物学(巢式PCR(nested PCR)、荧光定量PCR(FQ-PCR)、PCR-ELISA以及免疫PCR(I-PCR))诊断方法进行了概述,对其诊断的特点进行了比较和说明。

The diagnosis methods of Toxoplasmosis were summarized in lab,including pathogenic diagnosis(sample dyeing method,inoculate animal method,separate oocyst method and separate encyst method),immunology(Direct agglutination test,Indirect hemagglutination test,Modified agglutination test,Enzyme linked immunosorbent assay,Complement fixation test,Dye test,Indirect fluorescence antibody test) and molecule biology(nested PCR,FQ-PCR,PCR-ELISA,I-PCR),and diagnostic characteristic was compared and showed.